LOCUS       40924_42384        3111 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT7XbG2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Kikawada T, Kanamori Y, Saito A, Watanabe M, Okuda T.
  TITLE     Expression vector for Xenopus oocyte
  JOURNAL   Published Only in Database (2006)
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Kikawada T, Kanamori Y, Saito A, Watanabe M, Okuda T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2006) Takahiro Kikawada, National Institute of 
            Agrobiological Sciences; Ohwashi 1-2, Tsukuba 305-8634, Japan 
            (E-mail:kikawada@affrc.go.jp, Tel:81-29-838-6087)
REFERENCE   3  (bases 1 to 3111)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2006)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2006) Takahiro Kikawada, National Institute of 
            Agrobiological Sciences"; volume: " Ohwashi 1-2, Tsukuba 305-8634, 
            Japan (E-mail:kikawada@affrc.go.jp, Tel"; pages: "81-29-838-6087"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           659..687
                     /note="Xenopus beta-globin 5'-UTR, sub-cloned from
                     pXbG-ev1"
     misc_feature    687..699
                     /note="contains multiple cloning site (BgI II, EcoR V, EcoR
                     I)"
     3'UTR           722..847
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     promoter        complement(923..941)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(962..978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(986..1002)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1010..1040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1055..1076)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1364..1952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2126..2983)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2984..3088)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 gctcagaata aacgctcaac tttggcagat ctgatatcga attcggatct ggttaccact
      721 aaaccagcct caagaacacc cgaatggagt ctctaagcta cataatacca acttacactt
      781 tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg ctcctaataa aaagaaagtt
      841 tcttcacatt ctaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaacccc cccccccccc
      901 ccccgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
      961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1801 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
